Yeast Expression can be advantageous especially for the production of eukaryotic recombinant proteins. The system (Pichia
pastoris) take less than 10 weeks from subcloning to production. Recombinant Yeast protein production service includes:
- cloning and expression of interesting proteins
- optimization of production levels
- scale up from flask to fermenter, from laboratory conditions to industrial conditions.
Phases:
- Amplification/isolation of the gene of interest (GOI) from a customer-supplied vector and subcloning into
a suitable expression vector.
- Verification of the subcloned gene by restriction digest and sequencing.
- Gene cloning and transformation of a proprietary P. pastoris strain.
- Corroboration of the insertion sequence.
- Test of production and convalidation by SDS-PAGE and Western Blot
- Production on a scale of 1-3 liters and optimization of the downstream process (purification protocol of
the protein produced in native or denatured conditions).
Deliverables:
- Recombinant ptroteins
- Documentation
The solubility of the recombinant protein is not guaranteed.